产品货号Reference:               CAN-PRE-4500
检测方法Kit Type:                  Competitive ELISA
规格Kit Size:                          96-well break-apart microplate
灵敏度Sensitivity:                 0.05 ng/mL
检测样本Sample Type:         Human serum / 50 μL
校准范围Calibrator Range:  0.1–25.6 ng/mL
检测时间Total Assay Time:  75 minutes

背景介绍：

孕烯醇酮(3β-Hydroxypregn-5-en-20-one)是甾体合成途径中第一个由胆固醇衍生而来的甾体，是所有肾上腺和性腺类固醇的共同前体。它的产生是通过P-450SCC酶切割胆固醇C-20侧链而在线粒体中产生的。孕烯醇酮一旦产生，可通过两条甾体合成途径加以利用。孕烯醇酮可以通过17-α-羟化酶的酶促作用转化为17-OH孕烯醇酮，也可以通过3-β-羟基类固醇脱氢酶的酶促作用转化为孕酮。
Pregnenolone (3β-hydroxypregn-5-en-20-one) is the first steroid to be derived from cholesterol in the pathway of steroidogenesis, and it is the common precursor for all of the adrenal and gonadal steroids. Its production occurs in the mitochondrion by cleavage of the C-20 side chain of cholesterol by the P-450SCC enzyme. Once produced, pregnenolone may be utilized by two pathways of steroidogenesis. Pregnenolone may either be converted to 17-OH pregnenolone via the enzymatic action of 17α-hydroxylase or to progesterone via the enzymatic action of 3β-hydroxysteroid dehydrogenase.