产品分类PRODUCT
联系我们CONTACT
联系人:丁经理
电 话:0755-26055215
手 机:13265824656
传 真:0755-86372180
邮 箱:order@otwobiotech.com
地 址:深圳市南山区创业路中兴工业城5栋518
网 址:www.otwobiotech.com
您当前的位置: 网站首页 > 产品展厅 >抗体 >Human IgE抗体
Human IgE抗体
物品单位 品牌
Abbiotec
  • 产地:美国
  • 货号:250205
  • 发布日期: 2021-01-15
  • 更新日期: 2021-01-15
产品详细说明
CAS编号
抗体名 Human IgE
是否单克隆
靶点
用途范围 B E WB
形态 Each vial contains 1 mg IgE in 1 ml (1 mg/ml) of PBS pH7.4. No sodium azide. Antibody was purified by Protein-L affinity chromatography. 1 mg Human IgE corresponds to 380,000 ± 50,000 IU/ml.
宿主 Rabbit
标记物
纯度 %
免疫原 This is a non-immune hybridoma.
产地 美国
品牌 Abbiotec
货号 250205
保存条件 -20℃
应用范围 B E WB
抗原来源
保质期 一年
规格 1mg
产品用途 科研
克隆性
适应物种 H
亚型 Human IgE
标识物
浓度 %
是否进口

产品背景描述

This product is purified human IgE with kappa light chains produced in vitro from a hybridoma. Plasma IgE concentrations are low in normal patients. Elevated IgE levels are found in patients presenting severe allergic reactions and parasite infections. Original material was obtained from a healthy donor tested negative for HIV, HCV and hepatitis B by using US FDA-approved tests. Purity of human IgE is > 98% by SDS-PAGE analysis. There is no contamination by antibodies of other isotypes because of the cell line origin. Contaminants from foetal bovine serum were removed by Protein-L affinity chromatography. The material must be handled as potentially infectious as all human material. The product can be used as an IgE control in quantitative assays and for cell sensitization assays.



别名Alternate Names 

Immunoglobulin E; IgE


抗原Antigen 

This is a non-immune hybridoma.


应用说明Application Notes 

Product Cat. No. 250205 can be coated at 1-10 ug/well for ELISA assay format. Purified human IgE with kappa light chain is produced in vitro from a monoclonal hybridoma. Original material was obtained from a healthy donor tested negative for HIV, HCV and hepatitis B by using US FDA-approved tests. Purity of human IgE is >98% by SDS-PAGE analysis. Endotoxin level < 25 EU/mg.


引用文献Product Citations 

[1] Akache B. et al. 2016. Vaccines. 4(1): E3. PMID 26805897. [2] Weeratna R et al. 2016. Immun Inflamm Dis. 4(2): 135-47. PMID 27957325. [3] Sharquie I et al. 2013. BMC Immunol. 14:54. PMID 24330349. [4] Hideshima S et al. 2013. July. Electrochimica Acta. doi:10.1016/j.electacta.2013.07.113