产品分类PRODUCT
联系我们CONTACT
联系人:丁经理
电 话:0755-26055215
手 机:13265824656
传 真:0755-86372180
邮 箱:order@otwobiotech.com
地 址:深圳市南山区创业路中兴工业城5栋518
网 址:www.otwobiotech.com
猪免疫球蛋白G(IgG)ELISA检测试剂盒
物品单位 品牌
Genemed Synthesis Inc.(GSI)
  • 产地:美国
  • 货号:EK-9020
  • 发布日期: 2019-11-26
  • 更新日期: 2019-11-26
产品详细说明
产地 美国
品牌 Genemed Synthesis Inc.(GSI)
货号 EK-9020
检测方法 ELISA
CAS编号
规格
检测限 见说明书
数量 96T/盒
用途范围
标记物 见说明书
纯度 %
样本 血清
应用 ELISA
是否进口

EK-9020 猪免疫球蛋白G(IgG)ELISA检测试剂盒Pig IgG (total) ELISA Kit, 96 tests, Quantitative
产品介绍:
The Pig IgG ELISA kit is based on the binding of pig IgG in samples to two antibodies, one immobilized on the microtiter wells, and the other conjugated to horseradish peroxidase (HRP) enzyme. After a washing step, chromogenic substrate is added and color is developed by the enzymatic reaction of HRP on the TMB substrate, which is directly proportional to the amount of IgG present in the sample. Stopping Solution is added to terminate the reaction, and absorbance at 450nm is then measured using an ELISA microtiter well reader. The concentration of IgG in samples and control is calculated from a curve of standards containing known concentrations of pig IgG.                                                                        

测试原理

酶免测定法原理遵循典型的竞争抑制机制。存在于标准样本,对照样本和受测样本中的未标记的抗原与酶标抗原结合物之间相互竞争,争夺与事先包被在微孔板上的数量有限的抗体发生免疫反应的机会,然后通过洗涤和澄清去掉未被结合的物质。洗涤后再加入酶底物,*加入终止液终止酶促反应。吸光度可以通过酶标仪读取。形成的颜色的强度将与被检测的样本中的皮质醇的浓度成反比。
应用一套标准绘制标准曲线,从曲线上直接读取到受测样本和对照样本中的含量。

另有EK-9080 猪IgM检测试剂盒供应:

Pig IgM ELISA Kit, 96 tests, Quantitative

产品介绍:

Pig IgM ELISA kit is based on binding of Pig IgM from samples to two antibodies, one immobilized on the microtiter well plates, and other conjugated to the enzyme horseradish peroxidase. After a washing step, chromogenic substrate is

added and colors developed. The enzymatic reaction (color) is directly proportional to the amount of IgM present in the sample. Adding stopping solution terminates the reaction. Absorbance is then measured on a microtiter well ELISA reader at 450 nm. and the concentration of IgM in samples and control is read off the standard curve.


MATERIALS AND EQUIPMENT REQUIRED

Adjustable micropipet (5-1000ul) and multi-channel pipette with disposable plastic tips. Reagent troughs, plate washer (recommended) and ELISA plates Reader.   



注意事项
本检测试剂盒只能作为研究专用,请勿作诊断或其他用途!