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cat igg (total) elisa kit
| 物品单位 | 品牌 |
|---|---|
| 盒 | Genemed Synthesis Inc.(GSI) |
- 产地：美国
- 货号：EK-300-100-CGG
- 发布日期： 2019-08-07
- 更新日期： 2023-03-23
产品详细说明
| 产地 | 美国 |
| 品牌 | Genemed Synthesis Inc.(GSI) |
| 货号 | EK-300-100-CGG |
| 检测方法 | ELISA |
| CAS编号 | |
| 规格 | |
| 检测限 | 见说明书 |
| 数量 | 96T/盒 |
| 用途范围 | |
| 标记物 | 见说明书 |
| 纯度 | % |
| 样本 | 血清 |
| 应用 | ELISA |
| 是否进口 |
EK-300-100-CGG Cat IgG (total) ELISA Kit, 96 tests, Quantitative
操作步骤：
Step 1. Pipet 20 ul stds, pre-diluted samples (20 ul; 1:25k-100k). Dispense 80 ul of zero stds. into each well. Mix gently. Cover the plate and incubate at room temp. for 60 min.
Step 2. Aspirate and wash the plate three times.
Step 3. Add 100 ul of 1x HRP-labeled anti-Cat IgG conjugate. Mix gently, cover the wells, and incubate at room temperature for 30 min
Step 4. Aspirate and wash the plate five times.
Step5. Add 100 ul of TMB solution. Mix gently, cover the wells, and incubate at room temperature for 15 min
Step 6. Pipet 100 ul of stop solution into each well and mix gently (blue color turns yellow). Measure Abs. at 450 nm.
操作步骤：
Step 1. Pipet 20 ul stds, pre-diluted samples (20 ul; 1:25k-100k). Dispense 80 ul of zero stds. into each well. Mix gently. Cover the plate and incubate at room temp. for 60 min.
Step 2. Aspirate and wash the plate three times.
Step 3. Add 100 ul of 1x HRP-labeled anti-Cat IgG conjugate. Mix gently, cover the wells, and incubate at room temperature for 30 min
Step 4. Aspirate and wash the plate five times.
Step5. Add 100 ul of TMB solution. Mix gently, cover the wells, and incubate at room temperature for 15 min
Step 6. Pipet 100 ul of stop solution into each well and mix gently (blue color turns yellow). Measure Abs. at 450 nm.
