EK-300-100-CGG Cat IgG (total) ELISA Kit, 96 tests, Quantitative
操作步骤：
Step 1. Pipet 20 ul stds, pre-diluted samples (20 ul; 1:25k-100k). Dispense 80 ul of zero stds. into each well. Mix gently. Cover the plate and incubate at room temp. for 60 min.
Step 2. Aspirate and wash the plate three times.
Step 3. Add 100 ul of 1x HRP-labeled anti-Cat IgG conjugate. Mix gently, cover the wells, and incubate at room temperature for 30 min
Step 4. Aspirate and wash the plate five times.
Step5. Add 100 ul of TMB solution. Mix gently, cover the wells, and incubate at room temperature for 15 min
Step 6. Pipet 100 ul of stop solution into each well and mix gently (blue color turns yellow). Measure Abs. at 450 nm.