EK-500-100-ECP 小鼠抗大肠杆菌蛋抗体ELISA试剂盒Mouse Anti-E. coli proteins Ig's ELISA Kit, 96 tests, Quantitative

产品介绍：
The Mouse E. coli ELISA kit is based on the binding of Mouse anti- E. coli in samples to E. coli HCP immobilized on the microwells, and anti- E. coli Ig antibody is detected by anti-mouse IgG+IgA+IgM-specific antibody conjugated to HRP(horseradish peroxidase) enzyme. After a washing step, chromogenic substrate (TMB) is added and color is developed by the enzymatic reaction of HRP on the substrate, which is directly proportional to the amount of anti-E. coli HCP present in the sample. Stopping Solution is added to terminate the reaction, and absorbance at 450nm is then measured using an ELISA microwell reader. The amount of Mouse Ig in samples is determined relative to mouse anti-E. coli reference calibrators.
小鼠大肠杆菌ELISA试剂盒基于样品中的小鼠抗大肠杆菌与固定在微孔上的大肠杆菌HCP的结合，并通过与HRP(辣根过氧化物酶)结合的抗鼠IgG+IgA+IgM特异性抗体检测抗大肠杆菌Ig抗体。洗涤步骤后，加入显色底物(TMB)，通过HRP在底物上的酶促反应显色，其与抗-E的量成正比。大肠杆菌HCP存在于样品中。加入停止溶液以终止反应，然后使用ELISA微孔读取器测量450 nm处的吸光度。样品中小鼠免疫球蛋白的含量是相对于小鼠抗-E测定的。

注意事项

本检测试剂盒只能作为研究专用，请勿作诊断或其他用途！