EK-6310 小鼠IgA ELISA试剂盒Mouse IgA ELISA Kit, 96 tests, Quantitative
产品介绍：
The Mouse IgA ELISA kit is based on the binding of mouse IgA in samples to two antibodies, one immobilized on the microtiter wells, and the other conjugated to
horseradish peroxidase (HRP) enzyme. After a washing step, chromogenic substrate is added and color is developed by the enzymatic reaction of HRP on the TMB substrate, which is directly proportional to the amount of IgA present in the sample. Stopping Solution is added to terminate the reaction, and absorbance at 450nm is then measured using an ELISA microtiter well reader. The concentration of IgA in samples and control is calculated from a curve of standards containing known concentrations of IgA.
小鼠IgA ELISA试剂盒基于样品中的小鼠IgA与两种抗体的结合，一种抗体固定在微滴定微孔板上，另一种结合到辣根过氧化物酶(HRP)酶上。在洗涤步骤之后，加入显色底物并通过HRP在TMB底物上的酶促反应显色，其与样品中存在的IgA量成正比。加入停止溶液以终止反应，然后使用ELISA微滴度测井仪测量450 nm处的吸光度。样品和对照中IgA的浓度是根据包含已知IgA浓度的标准曲线计算出来的。