产品分类PRODUCT
联系我们CONTACT
联系人:丁经理
电 话:0755-26055215
手 机:13265824656
传 真:0755-86372180
邮 箱:order@otwobiotech.com
地 址:深圳市南山区创业路中兴工业城5栋518
网 址:www.otwobiotech.com
小鼠谷氨酸脱羧酶67(gad67) 抗体试剂盒
物品单位 品牌
Genemed Synthesis Inc.(GSI)
  • 产地:美国
  • 货号:EK-660-110-G67
  • 发布日期: 2019-08-07
  • 更新日期: 2019-08-07
产品详细说明
产地 美国
品牌 Genemed Synthesis Inc.(GSI)
货号 EK-660-110-G67
检测方法 ELISA
CAS编号
规格
检测限 见说明书
数量 96T/盒
用途范围
标记物 见说明书
纯度 %
样本 血清
应用 ELISA
EK-660-110-G67 小鼠谷氨酸脱羧酶67(GAD67) 抗体试剂盒Mouse Anti-Glutamic Acid Decarboxylase 67 (GAD67) Ig's ELISA Kit, 96 tests, Quantitative
产品介绍:
GAD67 ELISA试剂盒基于夹心ELISA原理。试剂盒测量样品中存在的未知数量的GAD67。我们用纯化的小鼠GAD67抗体包被在微滴度板孔上,形成固相抗体。然后将GAD67添加到微孔中。一种抗体-抗原酶-抗体复合物,是通过加入HRP标记的结合抗体而制成的。然后加入TMB(3,3‘,5,5’-四甲基联苯胺)作为显色剂,给出蓝色。最后,通过添加硫酸溶液终止酶催化反应,并在450 nm波长下用分光度法测量颜色变化。然后通过将样品的ODD与标准曲线进行比较来确定样品中GAD67的浓度。
The GAD67 ELISA kit is based on the sandwich ELISA principle. The kit measures an unknown amount of GAD67 present in the sample. We used purified Mouse GAD67 antibody to coat on microtiter plate wells, resulting in formation of solid-phase antibody. GAD67 is then added to the wells. An antibody-antigen enzyme-antibody complex is made by adding a combined antibody labelled with HRP. TMB (3,3',5,5'-Tetramethylbenzidine) is then added as a visualizing agent which gives blue color. Finally, the enzyme-catalyzed reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GAD67 in the samples is then determined by comparing the O.D. of the samples to the standard curve.