EK-6380 小鼠IgM ELISA试剂盒Mouse IgM ELISA Kit, 96 tests, Quantitative
产品介绍：
The Mouse IgM ELISA kit is based on the binding of mouse IgM in samples to two antibodies, one immobilized on the microtiter wells, and the other conjugated to horseradish peroxidase (HRP) enzyme. After a washing step, chromogenic substrate is added and color is developed by the enzymatic reaction of HRP on the TMB substrate, which is directly proportional to the amount of IgM present in the sample. Stopping Solution is added to terminate the reaction, and absorbance at 450nm is then measured using an ELISA microtiter well reader. The concentration of IgE in samples and control is calculated from a curve of standards containing known concentrations of IgM.
ELISA的基础是抗原或抗体的固相化及抗原或抗体的酶标记。结合在固相载体表面的抗原或抗体仍保持其免疫学活性，酶标记的抗原或抗体既保留其免疫学活性，又保留酶的活性。在测定时，受检标本（测定其中的抗体或抗原）与固相载体表面的抗原或抗体起反应。

本公司另有EK-6370 小鼠IgE ELISA试剂盒Mouse IgE ELISA Kit, 96 tests, Quantitative供应。

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