EK-101-RCP 大鼠C反应蛋白(CRP)ELISA检测试剂盒Rat C-Reactive Protein (CRP) ELISA Kit, 96 tests, Quantitative
产品介绍;
大鼠CRP ELISA试剂盒基于样品中大鼠CRP与两种抗体的结合，一种固定在微孔板上，另一种与辣根过氧化物酶(HRP)结合。经过洗涤步骤后，添加显色底物，并通过HRP在TMB底物上的酶反应显色，这与存在于TMB底物中的CRP的量成正比样本。加入停止液以终止反应，然后使用ELISA微滴度井阅读器测量450 nm处的吸光度。根据含有已知CRP浓度的标准曲线计算样品和对照中CRP的浓度。
The Rat CRP ELISA kit is based on the binding of Rat CRP in samples to two antibodies, one immobilized on the microtiter wells, and the other conjugated to
horseradish peroxidase (HRP) enzyme. After a washing step, chromogenic substrate is added and color is developed by the enzymatic reaction of HRP on the
TMB substrate, which is directly proportional to the amount of CRP present in the sample. Stopping Solution is added to terminate the reaction, and absorbance at 450nm is then measured using an ELISA microtiter well reader. The concentration of CRP in samples and control is calculated from a curve of standards containing known concentrations of CRP.