DE-100580 红霉素检测试剂盒Erythromycin ELISA Test Kit (water, urine meat(beef,chicken and pork),fish and shrimp)
红霉素ELISA试剂盒是一种高度灵敏的竞争型试剂盒，用于测定水、尿液、肉类(牛肉、鸡肉和猪肉)、鱼和虾中的红霉素。

使用步骤：
Step 1. Pipet 50ul each standards and samples containing Dimetridazole (diluted as required). Pipet 100ul Antibody#1 to the wells and incubate it for 30min at 25 ℃.Aspirate and wash the plate 3 times.
Step 2. 150 ul of antibody#2-enzyme conjugate solution in duplicates into the wells. Seal the plate, mix gently and incubate at 25 ℃ for 30min.
Step 3. Aspirate and wash the plate five times.
Step 4. Add 100 ul of TMB substrate solution to all wells, mix gently, and incubate at 25 ℃ for 15 min in the dark for coloration.
Step 5. Pipet 100 ul of stop solution into each well and mix gently (blue color turns yellow). Measure OD at A450 nm. Calculate concentration of Erythromycin in each sample using the Erythromycin Standard curve.