DE-100550 特布他林ELISA检测试剂盒Terbutaline ELISA Test Kit (feed, meat/liver/kidney, milk, urine)
背景描述：
Terbutaline is a β2-adrenergic receptor agonist. It is used as a reliever in asthma allergies (bronchodilator) and as anti-contraction medication to delay preterm labor. Terbutaline is currently on the World Anti-Doping Agency's list of prohibited drugs for Olympic athletes. The side effects in adults include tachycardia, anxiety, nervousness, tremors, headache, hyperglycemia, hypokalemia, hypertension and, rarely, pulmonary edema. In fetus, tachycardia and hypoglycemia is observed.

操作步骤：
Step 1. Pipet 50ul each standards and samples containing Terbutaline (diluted as required). Pipet 100ul Antibody#1 to the wells and incubate it for 30min at 25 ℃.Aspirate and wash the plate 3 times.
Step 2. 150 ul of antibody#2-enzyme conjugate solution in duplicates into the wells. Seal the plate, mix gently and incubate at 25 ℃ for 30min.
Step 3. Aspirate and wash the plate five times.
Step 4. Add 100 ul of TMB substrate solution to all wells, mix gently, and incubate at 25 ℃ for 15 min in the dark for coloration.
Step 5. Pipet 100 ul of stop solution into each well and mix gently (blue color turns yellow). Measure OD at A450 nm. Calculate concentration of Terbutaline in each sample using the Terbutaline Standard curve.