EK-104-MCP 小鼠C反应蛋白(CRP)ELISA检测试剂盒Mouse C-Reactive Protein (CRP) ELISA Kit, 96 tests, Quantitative
产品介绍：
小鼠CRP ELISA试剂盒基于小鼠CRP与两种抗体的结合，一种固定在微滴度孔板上，另一种固定在辣根过氧化物酶上。在洗涤步骤之后，加入显色底物并显色。酶反应(颜色)与样品中存在的CRP量成正比。加入停止溶液终止反应。然后在450 nm处测量吸光度。并从标准曲线上读出样品和对照中CRP的浓度。
Mouse CRP ELISA kit is based on binding of Mouse CRP from samples to two antibodies, one immobilized on the microtiter well plates, and other conjugated
to the enzyme horseradish peroxidase. After a washing step, chromogenic substrate is added and colors developed. The enzymatic reaction (color) is directly proportional to the amount of CRP present in the sample. Adding stopping solution terminates the reaction. Absorbance is then measured on a microtiter well ELISA reader at 450 nm. and the concentration of CRP in samples and control is read off the standard curve.

参考文献：
1. Powell L et al (1979) Am J. Med. Technol. 87, 138,
2. Osmand, AP(1977) PNAS 74, 739,
3. Ash R et al (1983) J. Infec. Immunity 53, 89;
4. Hedlulnd et al(1947) Acta Med. Scan. 128, 579;
5. Kushner I et la (1978) J Clin. Invest. 61, 235;
6.Hedlund, P et al (1961) Acta Med. Scan. 169, 1;
7. Yocum S et al (1957) Arch. Intern.Med. 99, 74;
8. Dowling P (1972) in Multiple Sclerosis, AP, pp269;
9, Roantree RJ et al(1955) Arch Int. Med. 96, 674;
10. Morley JJ et al (1982) Ann NY Acad Sci 389, 406;
11. Claus DR (1976) J Lab. Clin. Med. 87, 120.