AE-200170-1 猪细小病毒抗体Elisa试剂盒Porcine Parvovirus Antibody ELISA kit, 96 tests Monitor Disease (Serum)

背景描述：

Porcine Parvovirus is also known as PPV. It is in Group II, a single stranded DNA, from the family Parvoviridae and its genus is Parvovirus. PPV is one of the most common causes of infectious infertility. It is a very strong virus which multiplies itself in the pig’s intestine without giving the pig any symptoms. PPV is a very difficult virus to remove from the pig’s environment. PPV has unique characteristics of being resistant to most disinfectants and being able to survive living outside of its host for a long period of time. PPV will show symptoms in pregnant pigs only if the pregnancy is for the first time during the first 55 days.

操作步骤：

步骤1. 每次预稀释阳性血清、预稀释阴性血清各100μl，对照(仅含样品稀释剂)，以及在复孔中含有PPV抗体(根据需要稀释)的样品。轻轻混合并在37℃孵育30min。

步骤2. 抽吸并清洗三次微孔板。在所有孔中加入100ul的酶结合物，轻轻搅拌，37℃孵育30min。

步骤3.  抽吸并清洗三次微孔板。在所有孔中加入50微升的每种基质A和B溶液，轻轻混合，在黑暗中室温下孵育10分钟。

步骤4.  将50μl的终止液放入每孔中，轻轻混合(蓝色变为黄色)。在A630 nm处测量OD。如果值大于0.4，则采样为阳性；如果值在0.2和0.4之间，则为疑似；如果值小于0.2，则为阴性。