AE-200130-1 猪伪狂犬病抗体Elisa试剂盒Porcine Pseudorabies Antibody ELISA kit, 96 tests Control Disease (Serum)

操作步骤：

Step 1. Pipet 100 ul of each pre-diluted positive serum, pre-diluted negative, control (containing only sample diluent),

samples containing Pseudorabies antibodies (diluted as required) in duplicated wells. Mix gently and incubate at 37 ℃

for 30 min.

Step 2. Aspirate and wash the plate three times. Add 100ul of enzyme conjugate to all wells, mix gently and incubate at 37

℃for 30 min.

Step 3. Aspirate and wash the plate three times. Add 50 ul of each substrate A and B solution to all wells, mix gently, and

incubate in the dark at room temperature for 10 min.

Step 4. Pipet 50 ul of stop solution into each well and mix gently (blue color turns yellow). Measure OD at A630 nm. The

samples are positive if the value is > than 0.42, skeptical if between 0.38 and 0.42 and negative if < than 0.38.

在试验中，加入稀释的对照血清和待检血清，经温育后，若样品中含有抗猪伪狂犬gE 基因蛋白的特异性抗体，则将与微孔板上抗原结合，经洗涤除去未结合的抗体和其他成分后；再加入酶标二抗，与微孔板上抗原抗体复合物发生特异性结合；再经洗涤除去未结合的酶结合物，在孔中加TMB底物液，与酶反应形成蓝色产物，加入HF溶液中止反应后，用酶标仪630nm波长测定各反应孔中的OD值。