产品背景描述

This product is purified human IgE with kappa light chains produced in vitro from a hybridoma. Plasma IgE concentrations are low in normal patients. Elevated IgE levels are found in patients presenting severe allergic reactions and parasite infections. Original material was obtained from a healthy donor tested negative for HIV, HCV and hepatitis B by using US FDA-approved tests. Purity of human IgE is > 98% by SDS-PAGE analysis. There is no contamination by antibodies of other isotypes because of the cell line origin. Contaminants from foetal bovine serum were removed by Protein-L affinity chromatography. The material must be handled as potentially infectious as all human material. The product can be used as an IgE control in quantitative assays and for cell sensitization assays.

别名Alternate Names 

Immunoglobulin E; IgE

抗原Antigen 

This is a non-immune hybridoma.

应用说明Application Notes 

Product Cat. No. 250205 can be coated at 1-10 ug/well for ELISA assay format. Purified human IgE with kappa light chain is produced in vitro from a monoclonal hybridoma. Original material was obtained from a healthy donor tested negative for HIV, HCV and hepatitis B by using US FDA-approved tests. Purity of human IgE is >98% by SDS-PAGE analysis. Endotoxin level < 25 EU/mg.

引用文献Product Citations 

[1] Akache B. et al. 2016. Vaccines. 4(1): E3. PMID 26805897. [2] Weeratna R et al. 2016. Immun Inflamm Dis. 4(2): 135-47. PMID 27957325. [3] Sharquie I et al. 2013. BMC Immunol. 14:54. PMID 24330349. [4] Hideshima S et al. 2013. July. Electrochimica Acta. doi:10.1016/j.electacta.2013.07.113